Coding
Part:BBa_K4164996:Design
Designed by: Bo Cheng Group: iGEM22_NAU-CHINA (2022-10-02)
mRFP-TEVsite-Ssr
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The stop codon of the BBa_J04450 was deleted and TEV protease cutting site and Ssr tag were added to the C-terminus of mRFP by homologous recombination.
Source
This sequence is composed of BBa_J04450, BBa_K3139011 and BBa_K416007.